֪Rc(din)Y
Yǰһ(g)r(sh)εČW(xu)rM(jn)һȫϵyĿYʹ҂Ч҂һ(li)W(xu)(xi)Yô֪YΌ(xi)Сʹij֪Rc(din)Ygӭxϣ܉ϲg
֪Rc(din)Y 1
((g)wĴ̼(hu )a(chn)һDŽ(dng)B(ti)^(gu))cm(ҲoB(ti)m(dng)ֲıoɫ)ɻzQ
﹤̰֣քeǻ:(μg(sh))l(f)ø
Ĺͬ|(zh)A(Ԫ)N(li)N(li)RNADNAнYʽY(Ǿa^;a^)zܴa
ԪغռrĶٵքeOCHNPSԪC
wПo(w)C}ĹܺãȱF¼t\ݔ½wFSּ(dng);ȱFؚѪwFSw(dng)Θɏs
ֲ:е|(zh)քeǣ֬|(zh);(dng):еҪ|(zh)Ҫ֬͵|(zh)^ֱԴҪԴԴԴ
|(zh)Yԭ(4(g))DNAYԭ(3(g))DNAYԭ(3(g))
СˮƽR¿ɿֱС0.2ļYСļ֧ԭw
|(zh)ĻԪCHONSԪ;ĻԪCHONPPԪ;ѪtԪCHONFe~GصԪCHONMgԪCHON;V|(zh)Ԫص(li)֬
ԭ˼c(din)Тٟo(w)Ĥʢڟo(w)ȾɫwۃHкwܼڳɷľǢzѭҎɢރHеĿz׃ǻͻ׃ߟo(w)ĤϵyYa^Bm;(dng)to(w)˺;(xin)wM(jn)Пo(w)ȡĤĺò
|(zh)W(wng)ĤϵycĤBcӺĤB߀c(xin)wĤB|(zh)M(jn)ۯBMbǻȼӹγɾĤС\ݔߠwM(jn)һӹͷ
ڵпwɔ_(ǵ)øԭȍuL(chng)(jng)^(gu)ĤԼYЃ|(zh)W(wng)ߠwͼĤ
Nк˻Ҫȏƽ|(zh)SCȷֻŷֳɼzͼ|(zh)zɷNʽvδֻֻ˥ѴΔԽļԽL(chng)˥̓ͬõĽY
ֻČ(sh)|(zh)ǻxԱ_DˮƽɻɂȷǾa^{ص
ȫָѷֻĵļаl(f)ĝ(dng):ȫԴСɷ֞ȫԼ((dng)̥)(ԭc)(zhun)(Ѫɼ);ֲ:_ȫԴСǣֳܾw;ȫԵ|(zh)AǼȺбNȫz|(zh)
ӰøٷٶȵøضAȵʹø׃ԵǏAߜ(dng)wøĻԲضӰ-ømض60
̵Ĺø?xn)øDNABø(cI);̵ĹøwSøzø(@ԭ|(zh)wr(sh)m˝ȵҺCȝBoԭ|(zh)w)ȵø((dng):)
ATPǼֱԴ|(zh)ڼȺcADPDܵ(dng)(dng)\ݔźͷտs(jng)ЙCϳɵ;ܵНBv;γATP(dng)Ǻúⷴ
D׃ɻSW(xu)ܕr(sh)ӹwˮKwoø(NADP+)ه(li)B(ti)~Ga(sh)(xi)ˮNADPHγɵăɂ(g)ʽ
߹ʵķ1L(chng)Õr(sh)g(һ݆)2ӹe(ֲg)3߹Ч(ٶ)
B܄(ˮͪƾ)ͨ^(gu)ĤĔUɢȑQĦǰٷ֝
vˮ\ݔˮֵĄ(dng)Ҳ\ݔxӵĄ(dng);ֲˮĄ(dng)߀Ǹ;Ӱvõǜض(ض)L(fng)ֲˮ(1%--5%)vȴr(sh)FˮֲL(chng)Ę־
ҪֲͬͬˮͬM(jn)ɲÇιM(jn)M(jn)йsˮ
o(w)ֳ(Ă(g))(dng)¡g(sh)ֲMB(dng)ֲָ̥g(sh)(yu)c(din)DZHă(yu)ԠֲMBă(yu)c(din)ǣȡBڶֳʸԄ(dng)Уٷֳ;o(w)ֲ;a(chn)ˎʳƷӄɫx(chng);˹N;Dֲ
ֲˮֺ͌xӵǃɂ(g)^(gu)עДٶȴС
w(li)|(zh)(li)ā(li)ԴҪʳ֬(li)ԴҪǸߵD
|(zh)wȲ܃ǼĽY|(zh)|(zh)Դ|(zh)Óֽܷ|(zh)Ól(f)ڣ|(zh)z^(gu)ոz뵰|(zh)|(zh)ֽ^(gu)I
wÿ횔zһĵ|(zh)ԭǵ|(zh)ǼĽY|(zh)|(zh);|(zh)wȲ܃;DòγзN(li)İ;|(zh)wÿ춼(횰ɫه(li)Ki)
^eȾɫwM(һͬԴȾɫw)ȾɫwM((g)wȵȾɫwN(li)͔Ŀ)ȾɫwM(磺ѼȾɫwMɣ22l+X);Ի(ˮ24)ĆαM12lȾɫwzϢ;˻M(wDNAyȫzϢ)ˆαM(24lȾɫwzϢ);
ЙCxP(gun)ϵ((lin)ϵƼs)D(֬ܵ|(zh));DЗl(ǹD׃?yu)֬ǿɴD׃?yu)ֻ֬֬D׃?yu)?;Ƽs(ֻǴxϵKr(sh)֬|(zh));ǴxĘм~
֪Rc(din)Y 2
1.W(xu)f(shu)Ľ^(gu)
(1)W(xu)f(shu)Ą(chung )ʼʩRǺʩ
(2)W(xu)f(shu)Ҫc(din)ǣһ(g)ЙCwһЄ(dng)ֲﶼɼl(f)(li)ɼͼa(chn);һ(g)ĆλԼcͬMɵw;¼ɏϼЮa(chn)
(3)W(xu)f(shu)Ą(chung )M(jn)Ҫxǣʾκ΄(dng)ֲɼɵĶf(shu)(dng)ֲ֮gһHP(gun)ϵ֮gHP(gun)ϵʾM(jn)Ҫr(ji)ֵ
2.Nӵļ
(4)ȻϵyČӴУMϵy(g)wNȺȺB(ti)ϵyȦ
(5)ֲϵyӴЛ](mi)Сϵy@(g)Ӵ
(6)ԭ˼c˼ı|(zh)^eПo(w)ԺĤļ
չ
ԭ˼wo(w)ԭ缚ļҪɷ(li)c|(zh)Y϶ɵĻ
ԭzϵ?tng)zҎ;ֳ^(gu)˻zϵ?tng)zҎ
ȻlԭĿz׃(li)ֻлͻ׃;Ŀz׃(li)лͻ׃ؽMȾɫw׃
ԭ˼缚ҪԶѵķʽM(jn)з;˼ķѷʽнzo(w)zp
(7)ܪĴxͷֳ^(gu)ֻĻM(jn)
չ
ٲ(li)ǼȲԭҲ
ڽMÿNĻλķNÓķNǺ
۲BֱBB鲡ķֳĻM(jn)
3.ĤϵyĽY
(8)ò(dng).t(sh)ܷxõļĤѼˮˮ(hu )M(jn)뼚Ѽqȵ|(zh)(li)@ӾͿԵõļĤ
(9)ĤҪ֬|(zh)͵|(zh)M߀(li)
չ
ʹĤ|(zh)M(jn)ܵ|(zh)dw
ڼĤcĤĻW(xu)MɴͬڲͬĤW(xu)|(zh)ĺвeĤ(li)ĺcĤҪ
(10)ĤĽYc(din)(dng)x^(gu)
(11)ڼĤһɼĤϵĵ|(zh)c(li)Y϶ɵǵDZDZcReP(gun)ϵͺƤǵбo͝
(12)ֲ:ڵĻW(xu)ɷҪwSغz
չ
ټڵijɷ(li)c|(zh)Y϶ɵĻ
ڳwSøzøȥֲ:
֪Rc(din)Y 3
1zy߀ԭԇ0.1g/mlNaOHҺ0.05g/mlCuSO4ҺMc߀ԭǰl(f)ɴutɫʹÕr(sh)עFF
2bMе֬KȾҺKȾҺǰߌ֬ȾSɫȾɼtɫ
3bMеĵ|(zh)psԇʹÕr(sh)ȼNaOHҺ2~3CuSO4Һɫj(lu )
4oȾɫwȾɫõȾɫđϻtҺ
5^(gun)DNARNAڼеķֲüG_tɷNȾɫȾɫDNAȾɾGɫRNAȾɼtɫ
6ԭ|(zh)ӰĤҺĤԼ@ɌĤ֮gļ|(zh)
7ǾGȾҺnj(zhun)һȾ(xin)wĻȾʹо(xin)wʬF{Gɫ
8ڷڵĺϳӹ\ݔͷ^(gu)P(gun)ļw|(zh)W(wng)ߠw(xin)w
9γҪͨ^(gu)Óˮsϵķʽ
10ҺȴڼҺȕr(sh)ֲ:l(f)|(zh)ڷxF;ҺСڼҺȕr(sh)ֲ:l(f)|(zh)ڷxďԭF
֪Rc(din)Y 4
һ W(xu)гҊ(jin)W(xu)Ԫؼã
1Cawȱ֮(hu )ܛѪҺCa2+͕(hu )鴤^(gu)߄t(hu )o(w)ѪҺеCa2+дM(jn)ѪҺ̵ÙccѪҺеCa2+ѪҺͲ(hu )l(f)ֲвٵԪһȱ۵ĽM(hu )ܵ
2FeѪtĽMɳɷȱ(hu )ȱFؚѪѪtеFeǶr(ji)Fr(ji)FDzõֲвٵԪһȱ۵ĽM(hu )ܵ
3Mg~GwĽMԪܶøļֲȱVr(sh)~׳F~}ʧG
4BM(jn)۵Ȱl(f)ͻ۹ܵL(chng)ȱֲ(hu )F(sh)
5Iנټصijɷȱ׃(hu )СY˕(hu )طԼנ[
6KѪ⛺^(gu)͕r(sh)(hu )FļԄ(dng)ɮʧ
7NNǘ~GATP|(zh)ͺıԪNֲwγɵĻﶼDzĻˮNֲwȿƄ(dng)ȱNr(sh)~~N~SNһNˮB(ti)ϵyI(yng)BһNW(xu)ԪˮB(ti)ϵy^(gu)NcPϕ(hu )ɸI(yng)BڵˮB(ti)ϵyеĸI(yng)BQ(chng)顰ˮAںB(ti)ϵyеĸI(yng)BQ(chng)顰ೱ(dng)wȱN(sh)Hȱٰ͕(hu )Ӱ푵(dng)wL(chng)l(f)
8PPǘ֬ATPıԪֲwȱP(pn)(hu )Ӱ푵DNAďƺRNADĶӰ푵ֲL(chng)l(f)P߀cֲúͺеf^(gu)ATPADPжPҲˮB(ti)ϵyI(yng)BһNԪֲȱP(pn)r(sh)~׳Fo~Gϼtɫt
9ZnijЩøĽMɳɷҲøĻͽzϳɫøкZn](mi)ZnͲܺϳȱZnO(png)ҵֲС~Yͅ~Y~׃Сgs
W(xu)гõԇ
1ԇ ɷ֣0.1g/ml NaOHҺ0.05g/ml CuSO4Һ÷ԇҺҺweٌϺԇyҺˮԡӟֱӼӟyҺд߀ԭtʴutɫ
2Ƕԇ{ɫҺǻϺˮԡ(hu )FutɫǵĜy
3psԇɷ֣0.1g/ml NaOHҺ0.01g/ml CuSO4Һ÷yҺȼ2mlҺuм3~4Һuyдڵ|(zh)tʬFɫ
4K÷ȡKw95%ľƾuڙzy֬Ɍ֬ȾSɫKȾɼtɫ
5bDNADNAˮ(hu )Ⱦ{ɫ
6GbDNADNAGأ(hu )Ⱦ{Gɫ
750%ľƾҺ֬bKȾҺȾɫ50%ľƾҺϴȥɫ
875%ľƾҺښ75%ľƾܝB뼚ʹ|(zh)׃@(g)ƾĝBÓˮÜp@(g)t(hu )ʹ浰|(zh)ѸÓˮ̳ĤKƾ75ľƾҺg(sh)ǰQˎᘾǰƤwÓԼCе
995%ľƾҺswe֔95%ľƾDNA
1015%}95%ľƾҺweϿڽx
11đҺ(Ȟ0.01g/ml0.02g/ml)Ⱦɫw(zh)ɫɌȾɫwȾɫͨȾɫ3~5ҲôtȾɫ
1220%ĸK3%^(gu)3.5%ȻFڱ^^(gu)øFe3+ĴЧrĸKк^(gu)ø
133%ĿԵҺ3%Һ2%røҺ̽øۺǵÌ(sh)
14Һb۵Ĵ׃{
15ͪȡ~Gwеɫ
16Һɷ֣20ʯ2ݱͪ1ݱ϶Ҳ93̖ͣɫصČɫڞVϷx_(ki)
17裺ɫصȡķx(sh)ĥGɫ~Ƭr(sh)ʹĥ
18̼}ĥGɫ~Ƭr(sh)кЙCֹĥr(sh)~GwеɫƉ
190.3g/mLҺஔ30%Һֲ:Һĝȴ|(zh)ڷx(sh)
200.1g/mLęcҺcuѪѪ
21ȻcҺٿܽDNAȻcȞ2mol/L 0.015mol/Lr(sh)DNAܽȻcȞ0.14 mol/Lr(sh)DNAܽڝȞ0.9%r(sh)}ˮ
22ȵøٿÁ(li)ֽ⵰|(zh)ڿڄ(dng):Br(sh)ֽMʹMɢ
23ˮأ˹TwԇȰl(f)ķNӻʹȾɫwMӱԭǿڷѵļNwγ
24Ȼ}Ӽڵͨԣڻ̵Dʹ̎ڸܑB(ti)
֪Rc(din)YСP(gun)£
αһ֪Rc(din)Y02-19
Y03-16
tlj(mng)֪Rc(din)Y12-12
tlj(mng)֪Rc(din)Y12-11
γ֪Rc(din)Yͨ13ƪ08-30
Z(y)֪Rc(din)֮Z(y)R06-20
֪Rc֪Rc(din)07-28
ˮGij֪Rc(din)10-19
tlj(mng)֪Rc(din)12-20